Membrane Particles Derived From Adipose Tissue Mesenchymal Stromal Cells Improve Endothelial Cell Barrier Integrity
نویسندگان
چکیده
Proinflammatory stimuli lead to endothelial injury, which results in pathologies such as cardiovascular diseases, autoimmune and contributes alloimmune responses after organ transplantation. Both mesenchymal stromal cells (MSC) the extracellular vesicles (EV) released by them are widely studied regenerative therapy for endothelium. However, therapeutic application, manipulation of living MSC large-scale production EV major challenges. Membrane particles (MP) generated from may be an alternative use whole or EV. MP nanovesicles artificially membranes possess some properties MSC. In present study we investigated whether conserve beneficial effects on cell repair processes under inflammatory conditions. were hypotonic shock extrusion membranes. The average size was 120 nm, they showed a spherical shape. two ratios (50,000; 100,000 per target cell) human umbilical vein (HUVEC) tested model inflammation induced TNFα. Confocal microscopy flow cytometry that within 24 hours >90% HUVEC had taken up MP. Moreover, ended lysosomes HUVEC. co-culture system monocytes TNFα activated HUVEC, did not affect monocyte adherence but reduced transmigration across layer 138 ± 61 microscopic field 45 monocytes. stimulation 2-fold increase permeability monolayer measured translocation FITC-dextran lower compartment transwell system. At dose 1:100,000 significantly decreased (1.5-fold) respect Stimulated Finally, enhanced angiogenic potential vitro Matrigel assay stimulating formation structures, percentage covered area, total tube length, branching points, loops. conclusion, show cells, opening new avenue treatment vascular diseases where damage
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ژورنال
عنوان ژورنال: Frontiers in Immunology
سال: 2021
ISSN: ['1664-3224']
DOI: https://doi.org/10.3389/fimmu.2021.650522